Page 207 - Resúmen - XXV Congreso Latinoamericano de Parasitología - FLAP

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Candida albicans and Candida non- albicans in oral microbiota in health

young adults from North of Mexico

Rojas, Olga C ; García Tovar, Laura ; Amaral Montesino, Cintia ; Mendoza Olazarán,
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Soraya ; Carrión- Alvarez, Diego ; Morales- Ramírez, Paola ; Montoya, Alexandra M
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1 Universidad de Monterrey.; Universidad Autónoma de Nuevo León
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Candida is a member of the human microbiota and often resides on the oral cavity as part of the
microbiome. Candida albicans is the most frequent fungi isolated in healthy and diseased individuals.
Currently the recognition of the role of non-albicans Candida species as commensals has increased,
however very little is still known about this relationship in health and disease. The aim of this study was to
investigate the prevalence of Candida species in the oral cavity of healthy young adults aged 20 to 24
years, in Monterrey, Mexico. Samples were taken from both oral cheecks to a total of 240 students.
Furthermore we identified Candida species phenotypically by Gram stain and carbohydrate assimilation
test, CHROMagar and germinal tubes and confirmed the molecular identification using the primer internal
transcriber spacer (ITS5-ITS4). Enzymatic essays for hemolysin, deoxyribonuclease, phospholipase and
esterase were also performed. From the total of samples collected 59 resulted positive for Candida spp.
(24.5%). The yeast most isolated was Candida albicans with 88.1% (52/59), followed in frequency order
by C. tropicalis with 5.08% (3/59) and C. krusei with 3.38% (2/59) and just one strain of C. parapsilosis and
one of C. glabrata. A total of 21.6% (52/240) of the examined students resulted positive for C. albicans and
just 2.9% (7/240) gave positive results for non albicans species. Upon analyzing the enzymes there were
no strains capable of producing DNAse and Esterase, the presence of hemolysin and phospholipase was
not significant between albicans and not albicans group. Therefore we can conclude that non-
albicans Candida and C. albicans function as equal part of the microbiota and do not express significant
enzyme factors; In addition we will continue this research to determine in a larger scale, if non-albicans
species represent a health risk factor.

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