Page 255 - Resúmen - XXV Congreso Latinoamericano de Parasitología - FLAP

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Multidrug- resistant Escherichia coli isolates

from poultry farms in Ica, Perú

Davalos-Almeyda, Maria ; Guerrero, Agustin ; Medina, German ; Salvatierra, Guillermo ;
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Dávila-Barclay, Alejandra ; Calderón, Maritza ; Tsukayama, Pablo
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1 Universidad Nacional San Luis Gonzaga de Ica / Perú; Universidad Nacional San Luis Gonzaga de
Ica/Facultad de Medicina Veterinaria y Zootecnia; Laboratorio de Genómica Microbiana, Laboratorio de
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Investigaciones y Desarrollo, Universidad Peruana Cayetano Heredia; Laboratorio de Investigación en
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Enfermedades Infecciosas, Laboratorio de Investigaciones y Desarrollo, Universidad Peruana
Cayet; Laboratorio de Genómica Microbiana, Laboratorio de Investigaciones y Desarrollo/Universidad
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Peruana Cayetano Heredia

Introduction: Poultry farming is one of the main food animal production industries in Peru. However, the
common practice of using antibiotics as growth promoters exerts selective pressure on the microbial
populations that inhabit the intestines of chickens. Escherichia coli is the most common intestinal
commensal in vertebrates, and the emergence of multidrug-resistant (MDR) strains represents a risk to
animal and public health. Thus, Objetive: the purpose of this study was to determine the patterns of
antibiotic resistance of broiler chicken E. coli isolates in a region with a large poultry meat production in Ica,
Peru. Materials and Methods: Cloacal swabs were taken from 50 chickens from three farms during the
months of April, July and December 2018. The samples were placed in Cary-Blair transport medium and
refrigerated until processing. Isolation was done in MacConkey Agar and colonies were selected for
biochemical identification. Resistance to Chloramphenicol, Colistin, Meropenem, Nalidixic Acid,
Ciprofloxacin, Gentamicin, Azithromycin, Trimethoprim/Sulfamethoxazole, Tetracycline, Amoxicillin,
Cephalothin, and Cefepime was determined by disk diffusion test. Results: A total of 107 E. coli isolates
were identified and 89.7% (n=96) of MDR strains were detected. Resistance higher than 60% was
evidenced for Chloramphenicol, Tetracycline, Trimethoprim/Sulfamethoxazole, Amoxicillin, Cephalothin,
Nalidixic Acid, and Ciprofloxacin. Only one isolate was resistant to Colistin, and no resistance to
Meropenem was found. Conclusions: E.coli isolates from chickens intended for human consumption
showed high levels of resistance to different antibiotics for clinical use. Even, resistance to Colistin was
detected, a drug of last resort used for the treatment of severe infections caused by MDR strains. The
results highlight the need to implement measures to reduce the use of antibiotics in poultry systems in Ica,
Peru.

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