Page 254 - Resúmen - XXV Congreso Latinoamericano de Parasitología - FLAP

S1-67

Chikungunya and Zika natural and experimental infection rates by

molecular and immunological techniques in animals: a systematic review

and meta- analysis

Bonilla-Aldana, D. Katterine ; Jimenez-Cruz, Stephania ; Ramírez, Sebastian ; Rodriguez
2
1
2
Morales, Alfonso J.
3
1 Semillero de Zoonosis (SIZOO), Grupo de Investigación BIOECOS, Fundación Universitaria Autónoma de
las Américas, Pereira, Risara; Universidad Tecnologica de Pereira; Universidad Tecnológica de Pereira
2
3
Introduction: Description of many arboviral diseases occurred in animals, including
alphaviruses/flaviviruses, such as chikungunya (CHIKV) and Zika (ZIKV). There is still a lack of studies
assessing its reservoir role, especially after intense epidemics in the Americas, particularly due to low-
power studies evaluating it. Objective: To assess the combined prevalence of CHIKV and ZIKV animal
infections. Methods: We performed a literature systematic review in six databases (Web of
Sciences/Scopus/PubMed/SciELO/Lilacs/Google Scholar) to assess the proportion of animals infected with
CHIKV and ZIKV (molecular and/or immunologically). A meta-analysis with random-effects model was
performed to calculate the pooled prevalence and viremia (standardized mean), and 95% confidence
intervals (95%CI). Measures of heterogeneity (Cochran's Q-statistic, I2 index, and τ2 test), were reported.
Subgroup analyses were conducted by arbovirus and animal classes. Results: Till July 1, 2019, the
literature search yielded 2639 articles (719 on CHIKV and 1920 on ZIKV), of which only 9 studies were
valid for analysis. The pooled PCR+ prevalence of CHIKV (N=192) was 30.1% [95%CI(17.5%-
42.7%);τ2=0.160;I2=87.94;Q=381.43; p<0.001], highest value 97.6% (Lithobates). For the pooled mean
peak CHIKV viremia (N=154) was 2.051 log10 PFU/mL [95%CI(1.720-2.382); τ2=1.230;I2=99.997;
Q=1453595.042;p<0.001], highest at Eptesicus fuscus (5.500;4.389-6.611) and Mesocricetus auratus
(5.500;3.484-7.516). The pooled CHIKV seroprevalence (N=715) was 38.3% [95%CI(31.6%-
45.0%);τ2=0.060;I2=87.967;Q=631.603;p<0.001], highest adjusted values were in Alligator mississi-
ppiensis (92.9%) and Thamnophis sirtalis (91.7%). The pooled PCR+ ZIKV prevalence (N=152) was 4.8%
[95%CI(1.0%-8.6%);τ2<0.001;I2=21.94;Q=2.562;p=0.013], highest value reported 9% (Callitrichidae). The
pooled ZIKV seroprevalence (N=6119) was 4.3% [95%CI(3.0%-5.6%); τ2=0.001;I2=90.495; Q=326.152;
p<0.001], highest adjusted values were in Cercopithecidae (50%) and Gymnocephalus cernua (50%).
Conclusions: While Aedes is critical for human CHIKV/ZIKV epidemics, they persist in sylvatic
transmission cycles involving animals. Studies concluded that the infection frequency and seroprevalence
for them is low among animals, however, approximately a third of animals, PCR-tested for CHIKV were
positive, with higher seroprevalence. For ZIKV levels were lower (PCR and serology). Then, more studies
are needed.

252
RESÚMENES DE CARTELES