Page 268 - Resúmen - XXV Congreso Latinoamericano de Parasitología - FLAP

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Characterization of the Th1 7 immune response in human cutaneous lesion

caused by Leishmania (Viannia) panamensis in Panama, Central America.

Gonzalez Carrion, Kadir Amilcar ; Calzada, Jose Eduardo ; Diaz, Rosendo ; Tomokane,
2
1
3
Thaise ; Gomes, Claudia ; Corbett, Carlos ; Saldaña, Azael ; Laurenti, Márcia
4
4
4
5
4
1 Universidad de Sao Paulo/Patología-FMUSP, Instituto Conmemorativo Gorgas de los Estudios de la
Salud/Investigación en Parasitología; Instituto Conmemorativo Gorgas de los Estudios de la
2
Salud; Hospital Santo Tomás/ Laboratorio de Patología; Universidad de Sao Paulo/Patología-LIM50-
3
4
FMUSP; Instituto Conmemorativo Gorgas de los Estudios de la Salud/Investigación en Parastiología,
5
Universidad de Panamá/Microbiología

Cutaneous leishmaniasis (CL) is an endemic disease in Panama, caused by Leishmania (Viannia)
parasites, which are characterized for causing ulcerated lesions on the skin. Th17 cells are known for
playing an important role in eliminating pathogens that are not adequately destroy by Th1 cells, however,
Th17 may play an ambiguous role in leishmaniasis disease because in some cases it can help with the
elimination of the parasites but in others, contributes in the pathogenesis of the disease by exacerbating
the lesions on the skin. Despite the increasing knowledge of immunopathological mechanisms that
contribute to disease progression, the role of Th17 during L. (V.) panamensis infection remains unclear. In
this study we characterize the Th17 immune response in skin lesions of patients with CL caused by L. (V.)
panamensis to understand the pathogenesis of this specie that is endemic in Panama. Biopsies (n = 46)
from panamanian patients with localized CL were collected and processed by usual histological
techniques. L. (V.) panamensis infection was proven by in vitro isolation and characterization of the
parasites by HSP70-RFLP. In situ Th17 immune response was evaluated by immunohistochemistry using
anti-CD4, anti- RoRγt, anti-IL17, anti-IL6, anti-IL23 and anti-TGF-β. Quantitative morphometric analysis
showed that the density of CD4+ cells were 914.5±51.76 cells /mm2, RoRγt+ 229,20±13,49 cells/mm2, IL-
17+ 859.8±70.66 cells/mm2, IL-6+ 273.2±15.89 cells/mm2, IL-23+ 669.8±34.73 cells/mm2 and TGF-β+
132.2±9.50 cells/mm2. Positive correlations were found between these markers. Although not significant,
an inverse correlation was observed between the amastigote forms with the number of RoRγt+ and IL-17+
cells. These data may suggest that Th17 cells could be helping to eliminate the parasite by the action of
IL17, but it could also play an important role in the development of the disease pathology in tissue infected
with L. (V.) panamensis. Supported by SNI-SENACYT, FAPESP 2014/50315-0; 2017/03141-5, CNPq and
LIM50.

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